Rodent Tail Vein Injections (Mice) Date Issued: July 7, 2011 Revision: 2 Page No: 4 . Unlike the tail vein route, the femoral vein cut down requires the use of anesthesia. 3. The Mouse Tail Illuminator efficiently illuminates and warms a mouse tail for easy injection or bleeding. Intravenous Injections Warm the mouse under a heat lamp or other heating device, being sure not to OVERHEAT the animal. Place the tail in large beaker filled with warm water (40C) for 30 seconds. You can use commercially available re-usable "Hot Button- hot pack" that instantly becomes hot once the button inside the pack is activated. Wipe tail . The more 72 acceptable alternative is to dilate the veins by immersing into warm water (40 to 45 C) (8). iii. Tail Vein Injection. . 7th Oct, 2013 Alexa Wu Claude Bernard. Apply 70% Ethanol wipes to tail and turn 45o for a visible presentation of tail vein. (15 to 25 cm) above the Restrain the animal in a restraint device if animal is not anesthetized. J Neurotrauma 2002;19:205-21. it is kept warm by a heat lamp or other heating device (e.g., Deltaphase pads). This may be done using a heating chamber, heating pad or a heating lamp, or dipping tail in warm water. The novel induction chamber for tail-vein injection of mice is made of a special polymethyl methacrylate (1cm; 60 120 50mm) with inlet and outlet adapters for fresh and waste anaesthetic gas. (D) Immobilizing the mouse with a restraining device. with a little modification. More then one animal can be heated at a time. A heating pad, heat lamp, or hot water was used to dilate the tail veins for injections. saphenous vein); c) tail vein; d) retro-orbital; e) jugular vein; f) submental. Intraperitoneal Injection : Intravenous Injection Subcutaneous Injection Mouse. b. Immerse frog in solution for 10-20 minutes. 4-4: The mouse tail was extended and placed parallel to the table. Once the vein has been located, disinfect the site of injection and insert the needle at slight angle. Mice were main-tained under isourane anesthesia during the injection, accumu-lation, and scanning periods. You can inject up to 400 ul in volume. A heat lamp may be used if the water is not adequately warming the tail for the injection. 6 If the needle is inserted properly, the blood in the vein should clear and injection of the solution should be without resistance. Title: Microsoft Word - SOP 7.12 - Tail Vein Injection in Mice.docx Author: Be careful of the length of time the mouse is left under the lamp. 3. Tail-Vein Injection . Keep the bevel of the needle facing up. Mice can be placed back into the original cage immediately after injection. Gene Expression in the Liver and in Tumors 0.05-0.2% (500-2000mg/L) solution. If enough blood is needed, larger amounts should only be used, but care must be taken . The mice are transferred to a holding device, (AIMS restrainer) . Posted January 21, 2022 Donna. Typically, a manual tail vein injection consists of warming the mouse tail followed by securing the tail in the injector's hand and locating the tail vein by eye. 2. Mouse Tail Vein Catheterization/Injection 5/6/08 . Wrap tail in an exam glove filled with warm water for 12 minutes iii. - Mouse cage with wire lid. Mouse Intravenous Injections (Tail Vein) 1 - The tail vessels will be dilated by placing it in warm water (37-40 C) or placing the entire animal under a heat . Shaver to remove mouse hair. The mouse, Mus musculus, is the most commonly used laboratory animal.One of the most common and efficient routes of administration or injection in mice is the intravenous (IV) route. Intravenous injections Intravenous injections are usually made into the dorsal tail vein. 3. Tail Vein Injections in the Mouse and Rat SOP Page 3 of 3 5. Mouse Metabolic Phenotyping Centers MMPC Protocols 09/29/14 2 of 3 page(s) a. . which has a built-in heat source to help keep the animal warm. DONOR CELLS: (Need 10 million/mouse) 1. 2. After anesthetic induction, the animals have their bladders emptied via a cred maneuver. Return to top QUESTIONS Ken Hays DLAR Training Coordinator 859-323-5697 H41C ken.hays@uky . Once the tail is heated to adequate temperature, the animal is ready for the procedure. 4-6: The cell suspension was injected by gently pushing on the syringe's plunger. between 3-8 separate litters. In most cases warming the tail with the aid of a heat lamp or warm compresses will increase obtainable . 3. Take mouse out of strainer and check for vitality signs (normal heartbeat and breathing). Inject the solution slowly so that it flows at a slow pace through the vein. Mouse restrainer Heat lamp or vessel with warm water 70% isopropyl alcohol 2" x 2" gauze RAT . Warm the tail by immersing it in warm water or placing the animal under a heat lamp. 73 74 Tail vein injection also requires the animal to be mechanically restrained, and this can cause Sliding doors keep mouse gently restrained. Lightly anesthetize the mouse with isoflurane gas. Illuminated slot for easy tail vein injection, etc. . 99. (weight ranging 17-28 )grams. Then make sure that they can be shaded under the cage insert, such that they don't get too hot. The depth of anesthesia is monitored via withdrawal response and respiratory rate. Immobilize the tail with gentle traction. Heat the mice up first with the heat lamp until they exhibit whisker-grooming behavior. Cleaning the tail with ethanol is of some help and also, as you said, warming the tail. dynamic Tail Vein Injectiong pDNA-coding LacZ (2 pDNA/2 mL saline/mouse) was administered into tumor-bearing mice hydrodynamic injection. If injection of unanesthetized animals is required, the animal is placed in an appropriate passive restraint device (e.g. Placing the mouse under a heat lamp, maximum wattage of 40 watt, for 3-5 minutes. IVIS imaging. Then, the distribution and magnitude of LacZ gene expression was evaluated using Fig. Prep the tail with 70% ethanol. When doing an IV injection using the tail vein it is very convenient to have one of the commercial restraint devices . The tail vein-illuminating ring comprises a light-emitting . a. b. 2. Scale for weighing mice. Intravenous (IV) "Lateral Tail Vein Rat" Supplies: * 1-3ml syringe * 25g Needle * Heat Source Max Volume 0.5ml. 2.4. Introduce a 27G syringe into tail vein to administer an injectate into mice. - 0.5 mL syringe with 27-G ( 1/2-in.) Some technicians place animals under heat lamps, but this is discouraged, especially in small rodents, as the animals are often overheated leading to additional trauma and discomfort. Use heat to dilate the tail veins which run laterally along each side of the tail. Lateral Tail Vein Volume: <0.5ml Needle: <23 Gauge Back Scruff Volume: <5-10ml Needle: <20 Gauge; Hamster; Skin slowly (20-40 L/second) while watching for clearing of the . Restrain the tail while occluding the vein with your nondominant hand. Warm the tail with a heat lamp or by immersing in warm water to dilate vessels. Fish: 0.0025-0.01% (25-100mg/L) solution. injection (refer to luciferin injection protocols). Intravenous Tail Vein Injection Needle size 25 - 30G x 13mm, maximum bolus volume 1% of animal's body weight. You should not feel any resistance if you insert the needle right into the vein. The tail vein is easier to see in non-pigmented rats. Attempt the injection starting at the middle or slightly distal part of the tail. For Vascular Access in Mice) . Solution must be buffered with sodium bicarbonate to a pH of 7.0-7.5. Method to quantify tail vein injection technique in "good" injection, 2 received an "ok" injections and only 1 ani- small animals. It is common practice to stimulate dilation of the tail veins in mice either by placing them in a warmer environment (e.g. via tail vein slowly (another 2-3 minutes). Wipe the tail of the mouse with an . You can also try ethanol (70%) and rub onto the tail before your injection. Position the heat lamp at an appropriate distance from the animal cage to provide warmth and vasodilation. The lateral tail vein is usually used and 0.1 - 2 ml of blood can be obtained per sample depending on the size of the rat, sampling frequency and scientific justification. Alternately, the tail can be warmed under a heat lamp. Lateral Tail Vein Injection in . At the indicated time post injection, mice were given 200 l luciferin (15 mg/ml) intraperitoneally. Tail Nick 1. The tail vein is easier to see in non-pigmented mice. Retro-orbital injections in mice oThis technique is a useful alternative to tail vein injection. A heating pad, heat lamp, or hot water was used to dilate the tail veins for . at 28-30 C) for up to 30 minutes, or by placing the tail in warm (30-35 C) water. For heating the tail of the mouse directly, restrain the mouse (i.e. (A) Reagents and the apparatuses needed for the injection. INJECTION: 1. vii. (C) Anesthetizing the mouse with ketamine. . Prepare bone marrow from donors. Cannulation in the tail does not sound like a good idea to me, especially . Position the mouse so one of the lateral tail veins is visible. Use the smallest gauge needle you can get,. Arm Length: 2 spring loaded arms each 18" lengths Table Clamp : Standard "U" screw clamp expands to fit bench up to 2.25" deep Weight: 2 lbs. Techniques for tail vein dilation: The following techniques may be used to increase blood flow on the tail vein of a mouse or a rat: 1) Use of a heating lamp* 2) Submerging the tail in warm water (no warmer than 40 o C/104 o F) * 3) Placing rubbing alcohol over the tail * Animals under a heat lamp must be under direct supervision and care must . Disinfect the site of injection and slightly rotate the tail to visualize the vein. In the case of femoral cut down, only two injections are possible due to making incisions in the veins limits repeated injections and the availability of only two femoral veins restricts this route. vector per mouse in sterile PBS or DMEM via tail vein. Swab the tail with 70% ethanol on a gauze sponge or swab. ,MSPP-TV-150STEA,MSPP-TV-150LGEA Soft Temp Reusable Veterinary Pads, Braintree Scientific On day 0, animals are placed under a heat lamp for 4-5 minutes, and cells are injected i.v. Six hours was chosen to allow for the . Injections can be made into either of these veins. Rapid injection of a high-volume (10%, vol/weight) solution of the naked plasmid DNA through the tail vein generates a hydrodynamic pressure that expands liver endothelium resulting in DNA uptake by the hepatocytes [18,19]. Take a mouse, held by the tail, and guide it rear-first into the mouse restrainer. o Warm water, a heat lamp, or hand warmers may be used to dilate the blood vessels. Tail vein injection in mice was performed as described by TE Gratsch et al. Injections via the retro-orbital vascular sinus do not depend . (E) Inserting the needle in the distal portion of the tail vein and injecting the DNA solution into the tail vein. For better visualization of the vein and dilation, warm up the vein to ~37C using a water bath or a heat lamp. The tail may need to be washed with diluted Hibiscrub (1%) in order to see the blood vessel. IV injections are most commonly performed in the tail veins. This will increase the blood supply to the tail, enlarging the veins and making them easier to locate and inject. CTKcom UVA UVB Light Bulb Reptile Ceramic Heat Lamp Pet Heating Bulb Holder Clamp Lamp Fixture Heating Light Lamp For Reptiles,Aquarium Reptile Light Adjustable Habitat Lighting Stand,110V-130V(Black) 4.0 out of 5 stars 433. c. - Mouse restrainer. The lateral (side) veins are immediately below the skin, and must be . Blood withdrawal by cardiac puncture is . Warming the mouse under a heat lamp helps to bring up the tail vein, but be careful not to heat stress the whole box as this may affect your results. Preparation for the surgeon: The surgeon wears a facemask, hair bonnet, surgical gown and clean . Hydrodynamic tail vein injection]. (Tail may be immersed in . In a sterile culture hood, collect 300 l of cell . As the mouse tail warms up, the vein should dilate and become more visible. 1. Warm the tail by immersing it in warm water or placing the animal under a heat lamp. This protocol details the fabrication and application of a 3D-printed device for facilitating lateral tail-vein injection in mice. LAB_030 Injections - Intravenous (IV) Tail Vein Injection in Mice and Rats Institutional author: UQ Biological Resources . To facilitate tail vein visualization and ensure optimal injections, dilate the tail vessels immediately prior to injection by warming the tail of the mouse with a safe, effective heat source (e.g. oThe mouse should be anesthetized so that it remains still during . With your left hand, hold onto the tail so that the vein is facing upward, facing you. We heated mice with a 50-W heat lamp until the lateral tail vein dilated. Briefly, mice were warmed for 5 min using a heat lamp and a heating pad, and then siRNA expression plasmid (pSilencer4.1/Sry565) or negative control plasmid (pSilencer4.1/EGFP) was mixed with or without PEI micelles . 1. When anesthesized (ketamine/xylazine or isoflurane) we keep our mice on a heated pad anyways. Lateral tail vein injection of mice for Mtb infection (A) The tail of the mouse can be warmed using methods such as a light source, warm water, or a red heat lamp for vasodilation and ease of. . While holding the syringe in your right hand (if right-handed), carefully inject the needle into the vein, while still holding onto the vein so that it does not roll. Do not keep mice in the heat box for more than 20 min. Place mice to be injected (up to 6 mice) in cage and shine a heat lamp ~6 to 10 in. View the tail vein sampling technique below. 1 - The tail vessels will be dilated by placing the tail in warm water (37-40 C) or placing the entire animal under a heat lamp (at least 25-30cm from the bulb) for 2-5 minutes. Insert the needle at a slight angle and inject . A fine gauge needle should be used for this procedure. If a warming box or incubator is used, its temperature should be monitored carefully. With the torso in the main chamber of the restrainer, with the . Intravenous injection. Visualize the lateral tail vein and insert needle parallel to the vein 2 to 4 mm into the lumen. warm water (~37C) or heat lamp (120 W bulb)) for 3-5 minutes. needle info@signagen.com Injection Procedures: 1. 4. Transfer mice to a restrainer with a hole for the tail. . A second scan was performed with a repeat injection of 18 F-FDG approximately 6 h after the first injection. - Heat lamp. A mouse model of acute ischemic judged to have received an "excellent" injection, 3 received a spinal cord injury. Place tail on heating pad and cover with hand iv. vi. 4. 3. Slightly rotate the tail to visualize the vein, and once located, disinfect the injection site using an alcohol swab. the left and right lateral tail veins, in the ~10 and 2 o'clock positions (Figure 18). 3. Clean the tail with 70% alcohol. The rodents are initially put under a heat lamp to increase blood flow to the tail vein. 3. Mouse Tail Vein Injection (JK Lab 2006) Following step IV.c.4, prick the vein perpendicular to the tail as close to the tail tip as possible. For a better visualization of the vein and dilation, warm up the vein to ~37 C using either a water bath or a heat lamp. - Cotton gauze pads. Some crazy procedures allow you to do 1.5 ml. We are a women owned small business, dedicated to providing new product technology and supplies to advance medical milestones for all. tunnel restrainer). Inject 150 ml cells (10 million) i.v. Here I introduce a special 180 LED illumination device to improve the visibility of the tail veins of black mice and report on the application of a Broome style restrainer as well as the selection. Heat Lamp with table clamp. "Lateral Tail Vein Mouse" . Place mice in clean cages and keep warm with a heat lamp for ~5 min with constant monitoring to induce vasodilation. Get it as soon as Fri, Aug 19. for catheter placement or tail vein injection of radiotracer. You should be able to see the vein blanch if the needle is properly positioned. Mice were then warmed by heat lamp for 30 min to recover from injection shock. Mice and Rats (Preferred Technique . 3.7 MBq of FDG in 50-100 L of saline is . IV vascular access is technically challenging in the adult mouse. (iv) Placing the mouse in an incubator for 5 - 10 minutes set at 35oC. There are two veins in the mouse's tail, one on the ventrum of the tail. Best to heat the mouse for 2-3 min with a 200 W lamp to dilate the tail vein before injection. For image acquisition, the animal will be placed on plastic-backed absorbent paper to . To maintain the animal's body temperature, we use a heat lamp when the mouse is in the induction chamber and on the lab-built injection platform. Before injection the tail was immersed in a 39-40 C water bath for about a minute to cause sucient dila-tation of the lateral tail veins. Traditionally, the needle insertion is performed manually by hand, where success rates depend on technical skill [].The most common vein used for needle insertion in rodents is the lateral tail vein, which for a typical lab mouse and rat is approximately 0.3 and 0.5 mm in diameter, respectively [7,8].Due to the small size of the vessels, oftentimes, they are difficult to localize and puncture. 1. The pack remains hot for at least 30 minutes. Live animal imaging was done on the IVIS 200 machine (Caliper Life Sciences . place in a restrainer if conscious or anesthetize) and: i. Submerse the tail in warm water for 12 minutes ii. Level of anesthesia is judged by loss of righting reflexes, loss of gulping reflex and loss of withdrawal response to toe pinch. Stabilize the tail between the thumb and forefinger of the hand that will not be manipulating the syringe. Ensure good hemostasis by applying pressure with a dry gauze pad; clotting agents or surgical glue may be used if needed. Isolate the mouse in a small cage or container. Place mouse in a restrainer. Heat the mouse with an infrared lamp. Warming the mouse (or tail only) causes vasodilation and provides better vein visibility. Identifying the . Mouse: Grasp the tail at mid-length or at the distal . The body temperature during all surgical procedures and during recovery from anesthesia is maintained using a heat lamp and/or heating pad. The heat lamp poses a high 71 risk of causing hyperthermia, heat distress and burn-related injuries to the animals. Try a heating pad to gently warm the tail and improve vasodilation. If an overhead heat lamp is used, extra care must be taken to prevent overheating the animal. Animals are anesthetized (another 2-3 minutes), then imaged. Mice received either retro-orbital or tail-vein injections daily for 5 d. On the day after the final injection, we killed. 10 Best Heat Lamp For Mouse Tail Vein Injection. 4. With the bevel of the needle facing upward and the needle almost parallel to the vein, slide the needle into the tail vein. Mouse 20 1.10 - 1.40 11 - 14 l 90 - 105 l 110 - 140 l 25 1.37 - 1.75 14 - 18 l 102 - 131 l 140 - 180 l . To keep the mouse steady during the injection, place it into a plastic restrainer, slipping the tail through a special hole in the back. The injector then uses their other hand to advance a 28 or 30 G needle into the mouse tail vein until a flashback of blood is observed. Each unit comes with a restraining tube and illumination source. Description: Tailveiner is the first easy-to-use mouse and rat restrainer specifically designed for tail vein injections. Additional tubes are available. ii. We sometimes put one of those flexible lamps (such as this) to heat up only the tail. If no anesthesia is used, transfer mouse into the barrel of the plastic or brass restrainer, which restrains the animal while allowing access to the tail vein. After a tail vein injection of 3.7-7.4 MBq of 18F-FDG in 200 mL of PBS, a 7-min prone acquisition scan was performed approximately 60 min after injection. 4-5: The needle was inserted into the tail vein from the distal end and the needle was held steady. Species: Mouse. Skin Volume: <0.05ml Needle: <25 Gauge Rear Leg/Thigh Volume: <0.05ml . Reagent setup Note: Heat lamps and heated surgery pads may also be used to induce tail vein dilatation. . The present invention relates to a kind of multi-functional tail vein injection platform, described tail vein injection platform includes platform body, mouse cage, regulator, rat-tail bracket, temperature regulating device, light-guiding system device, magnifying glassDescribed mouse cage is fixed on the upper surface of platform bodyThe front end of the mouse cage is provided with . View Varients. Collect blood via capillary action using hematocrit tube or allowing blood to drip into collection tube. Hydrodynamic Injection: 1. Braintree Scientific has been serving the needs of the Life Science industry since 1980. Figure 13: Intramuscular Injection Site Figure 14: Intramuscular Injection - 2 people Intramuscular Injection - Restrainer . First, mice are warmed under a heat lamp for a few minutes, which causes the veins to swell and makes needle insertion easier. Groman EV, Reinhardt CP. CAUTION: do not overheat; animal should be under a heat lamp no longer than three minutes with heat lamp at least 12 inches away. Restraining tube slides off base for easy cleaning. Warm the bottom of the mouse tail under heat lamp for 10 s. v. Rapidly inject mouse via the tail vein (within 5 to 7 s) with syringe held at a 10 degree angle to the vein. The veins of the tail should be easily visible. 2. BN-LINK Digital Heat Mat Thermostat Controller for Seed Germination, Reptiles and Brewing Breeding . Mice were maintained under isoflurane anesthesia during the injection, accumulation, and scanning periods. (B) Dilating the tail blood vessels with a heat lamp. through the tail vein. . Hydrodynamic injections were carried out as previously described [36] using a heat lamp for tail vein dilation, with the exception that sterile phosphate-buffered saline #20012 (Invitrogen, Carlsbad, CA) was used to dilute the DNA. $13.99 $ 13. 4. Intravenous injections Intravenous injections are usually made into the dorsal tail vein. Must inject slowly . . . Power: 110VAC Bulb: 250 watt infrared heat Lamp Infrared heat lamp is table mounted with an adjustable swing arm providing for easy adjustment of radiant heat to specimen. Tumors and livers via were collected 13 h after administration. 2. Warming the tail causes the veins to dilate. 45-50C waterbath and/or heat lamp. N.B. Keep bevel up and parallel to the rest of the tail (it will be going into the tail at an angle because the tail is being held over your forefinger, if that makes sense). They can be placed in a plastic cage, chamber, or bowl with the lamp above them. veins more apparent- wipe in the direction of the scales to avoid irritation to the tail. 1 It can be used to administer cells, chemical agents, infectious disease agents, drugs and any other reagent that needs a systemic delivery. Or transplant the stem cells by retro-orbital injection (easier at the beginning). 2. If the vein is not easily visualized or accessed, consider using another injection route, such as retro-orbital injection as described above. Tail Vein Injection 1. 22. o The tail vein is difficult to find that's why mouse is often placed under a heat lamp to promote peripheral vasodilation o Suitable for large volumes. Order and timing of injections and imaging: a. Luciferin is given first by i.p. Intravenous injection into the mouse lateral tail vein can be a challenging . If there is resistance upon pushing the plunger, the needle is not placed properly into the vein. They are. The cap has . Heat lamps or heated surgery pads, while not necessary, will also help by increasing the volume of the tail vein. Histology and immunohistochemistry. A fine gauge needle should be used for this procedure. This procedure has the risk of needle stick or mouse bite injury - take appropriate care. Technique: 1.
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